Cyto-id® green detection reagent

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August undefined, 2024

WebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the … WebAug 27, 2024 · Prior to staining, dilute 1 μL of CYTO-ID ® Green Detection Reagent in 1 mL of phenol red free complete cell growth medium containing 5% FBS. 5. Re-suspend the pellets in 250 μL of the diluted CYTO-ID ® Green stain solution, and incubate for 30 min at 37 °C in the dark (see Note 11). 6.

Interactions between autophagic and endo-lysosomal markers …

Web- 2 µL of CYTO-ID® Green Detection Reagent and 1 µL of Hoechst 33342 Nuclear Stain for every 1 mL of 1X Assay Buffer or complete cell growth medium: Downstream tips ; Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... WebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ... ece group otto

Enzo Life Sciences CYTO-ID Autophagy detection kit (1x200tests)

WebNovel Green Plus (20,000X) DNA Staining Reagent: 500 μl: AGT002-0500: Agarose Tablets, 110 pcs: 0.5g x 110: ... COVID-19 RT-qPCR Detection Kit Plus (TFDA) 100 rxns: ... 【CYTO-ID ®自噬檢測試劑盒】 Autophagy detection kit貨號ENZ-51031-K200 WebThe DYNEX Technologies Automated ELISA System is a cutting edge computer-controlled processing system that achieves full automation of ELISA assays and surrounding … WebFluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer Catalog No. 50-103-1813 $558.00 / Each Qty Check Availability Add to cart Provide Content Correction complicaties sectio

Advanced photodynamic therapy with an engineered M13

CYTO-ID ® Red long-term cell tracer kit - Enzo Life Sciences, Inc.

WebJul 16, 2014 · Cyto-ID Green Detection Reagent Kit (Enzo Life Sciences, Farmingdale, NY) detects autophagosomes and autolysosomes as recommended by manufacturer . In … WebThe CYTO-ID ® Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected … complicaties slechtziendheidWebApr 28, 2015 · A. parasiticus SU-1 was grown for 66 h (as described in Figure 1) in the presence of Rapamycin (500 nM, added at 24 h to induce autophagy). 6–8 colonies were stained by Enzo Cyto-ID ® Green Detection Reagent according to manufacturer’s protocol. The stained cells were observed under a fluorescent microscope as described in Methods. ece government

"WebIn brief, cells were washed with PCR PBS (supplemented with 5% FBS) and then Gene Primer sequence mixed with Cyto-ID Dual Detection Reagent solution (Cyto-ID Green Detection Reagent and GAPDH Hoechst 33342). " - Cyto-id® green detection reagent

Cyto-id® green detection reagent

Using Cellometer Vision and Cyto-ID Stain for Autophagy …

WebDec 1, 2024 · CYTO-ID® Green dye eliminates background staining of lysosomes seen with other lysosomotrophic dye-based assays that utilize monodansylcadaverine (MDC) (bottom panel). The CYTO-ID® … WebMay 4, 2024 · The pattern of vacuole labeling 4 h after ER photodamage in the presence vs. absence of 5 μM cycloheximide with ERTracker Green, a fluorescent probe for the ER, is …

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WebThe CYTO-ID ® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by … WebNov 8, 2024 · To determine the effects of LH or AICAR on mean fluorescence intensity of CYTO-ID® Green Detection Reagent, images were converted to maximum intensity projections and processed utilizing ImageJ (National Institutes of Health) analysis software.

WebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min.

WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature … WebCYTO-ID® Autophagy Detection Kit 2.0, Enzo Life Sciences Supplier: Enzo Life Sciences A brighter and more photostable, no-transfection, quantitative assay for monitoring …

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WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ... complicaties rokenWebOct 20, 2016 · The Cyto-ID ® Green Reagent is a cationic amphiphilic tracer dye which selectively labels pre-autophagosomes, autophagosomes, and autophagolysosomes. … complicaties slokdarmkankerWebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … ece group berlinWebThe CYTO-ID® Green Detection Reagent and the Autophagy Inducer (Rapamycin) contain DMSO which is readily absorbed through the skin. DMSO is harmful if ingested or … ece grant ohioWebCellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. … complicaties smetplekkenWebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … ece halilWebDec 1, 2012 · The Cyto-ID green reagent (Cyto-ID) is a. ... Lyso-ID Red Detection Reagent (Lyso-ID) were from Enzo. Life Sciences. Cell culture. Bovine aortic endothelial cells (BAEC) were obtained from. complicaties onderkoeling