Tīmeklis2024. gada 28. apr. · Next, 20 μL of this solution was added to the assay plate for a final concentration of 111 nM 5-HT (0.1% DMSO). The same 5 ROIs were imaged after 5 min of incubation. Next, 20 μL from the treatment plate was transferred to the assay plate for a final 1:1000 dilution of drug (10 μM drug, 100 nM 5-HT, 0.2% DMSO). Tīmeklis2024. gada 9. jūn. · For this, we inserted the RTK fragments consisting of their transmembrane domain (tmRTK) followed by the cytoplasmic domain (cytoRTK) between Igk-DrBphP–PCM and Golgi patch-FCYENEV motifs via ...
Yalan Han
Tīmeklis2024. gada 6. apr. · For mammalian neuronal expression, the coding sequence of QuasAr2 53 (Addgene #107705) was cloned together with a trafficking signal (ts: KSRITSEGEYIPLDQIDINV) and an ER-export signal (ER: FCYENEV ... TīmeklisAll plasmids in this study are derived from pEM705, which contains a CAG promoter for constitutive expression, obtained from E. V. Makeyev (Khandelia et al., 2011).Additional information regarding the plasmid and usage can be found in the Supplementary section of Ballister et al. (2014, 2015).. Halo-GFP-ActA (referred to as Halo-GFP-Mito) + … pseg buy thermostat
Addgene: hSyn ArcLightCo (Q239)-T2A-nls-mCherry
Tīmeklis2024. gada 25. janv. · The mechanisms underlying the effects of ultrasonic modulation of neural activity are unclear. Here, the authors show that focused ultrasound excites cultured primary murine cortical neurons via ... Tīmeklis2024. gada 25. janv. · Focused ultrasound robustly activates cortical neurons. To study neuronal responses to focused ultrasound under acoustic conditions matching soft tissue, we cultured primary murine cortical neurons on an acoustically transparent mylar film while optically recording their calcium and voltage responses to ultrasound using … Tīmeklis2014. gada 13. aug. · To enhance membrane trafficking of fusion proteins, we added a TS and ER export signal peptide sequence (FCYENEV), derived from the inward rectifier potassium channel Kir2.1, as previously ... horse stall front kits